@Article{096504017X15140544654946,
AUTHOR = {Jianping Xu, Liguo Sun, Wei Sun, Jianhai Tian, Huaiyuan Guo},
TITLE = {Targeted Silencing of <i>Kim-1</i> Inhibits the Growth of Clear Cell Renal  Cell Carcinoma Cell Line 786-0 In Vitro and In Vivo},
JOURNAL = {Oncology Research},
VOLUME = {26},
YEAR = {2018},
NUMBER = {7},
PAGES = {997--1003},
URL = {http://www.techscience.com/or/v26n7/56713},
ISSN = {1555-3906},
ABSTRACT = {To investigate the effect of <i>Kim-1</i> on 786-0 cells in vivo and in vitro, several experiments such as quantitative 
real-time PCR, Western blot, MTT, colony formation, and flow cytometry were performed to evaluate the biological behavior of 786-0 cells treated with <i>Kim-1</i> siRNA. Furthermore, the tumor xenograft model was applied 
to BALB/c nude mice to assess the effect of <i>Kim-1</i> silencing. Lentivirus-mediated RNAi effectively silenced 
<i>Kim-1</i> in 786-0 cells. <i>Kim-1</i> knockdown significantly inhibited the proliferation and colony formation ability 
of 786-0 cells (<i>p</i> < 0.01). The cell cycle of 786-0 cells was arrested in the G<sub>0</sub>/G<sub>1</sub> phase (<i>p</i> < 0.01). Early and late 
apoptosis were significantly increased in the <i>Kim-1</i> siRNA cells (<i>p</i> < 0.01). In addition, growth of 786-0 cells 
was significantly inhibited in the <i>Kim-1</i>-silenced mice. In conclusion, knockdown of <i>Kim-1</i> inhibits the growth 
of 786-0 cells in vitro and in vivo, indicating that <i>Kim-1</i> could be used as a potential target for clear cell renal 
cell carcinoma therapy.},
DOI = {10.3727/096504017X15140544654946}
}