@Article{096504018X15151486241153,
AUTHOR = {Wei Lei, Wang Kang, Yang Nan, Zhang Lei, Li Zhongdong, Li Demin, Sun Lei, Huang Hairong},
TITLE = {The Downregulation of miR-200c Promotes Lactate Dehydrogenase A  Expression and Non-Small Cell Lung Cancer Progression},
JOURNAL = {Oncology Research},
VOLUME = {26},
YEAR = {2018},
NUMBER = {7},
PAGES = {1015--1022},
URL = {http://www.techscience.com/or/v26n7/56715},
ISSN = {1555-3906},
ABSTRACT = {This study was aimed to investigate the function and mechanism of microRNA-200c (miR-200c) in the progression of non-small cell lung cancer (NSCLC). A total of 76 patients diagnosed as having NSCLC were 
enrolled in this study. The expression level of miR-200c in NSCLC tissues and cell lines was investigated using 
the quantitative real-time polymerase chain reaction (RT-qPCR) method. We found that the expression of miR-
200c was significantly reduced in NSCLC tissues and cell lines compared with normal lung tissues and the 
human bronchial epithelial cell line. Overexpression of miR-200c using the miR-200c mimic significantly suppressed cell proliferation and migration of NSCLC cell lines. The results of the luciferase reporter assay identified lactate dehydrogenase A (LDHA) as a direct target of miR-200c. The expression of LDHA was shown to 
be suppressed in NSCLC cell lines with miR-200c mimic transfection. Furthermore, the transfection of small 
interfering RNA (siRNA) targeting LDHA suppressed the proliferation and migration of NSCLC cell lines. In 
summary, our results presented in this study suggested that miR-200c was able to inhibit the proliferation and 
migration of NSCLC cells by downregulating LDHA. Therefore, miR-200c may be considered as a potential 
candidate for the treatment of NSCLC.},
DOI = {10.3727/096504018X15151486241153}
}