@Article{096504018X15164123839400,
AUTHOR = {Mengyi Huang, Xin Gong},
TITLE = {Let-7c Inhibits the Proliferation, Invasion, and Migration  of Glioma Cells via Targeting E2F5},
JOURNAL = {Oncology Research},
VOLUME = {26},
YEAR = {2018},
NUMBER = {7},
PAGES = {1103--1111},
URL = {http://www.techscience.com/or/v26n7/56725},
ISSN = {1555-3906},
ABSTRACT = {As a member of the miRNA family, let-7c has been identified as a tumor suppressor in many cancers. However, 
the molecular biological function of let-7c in glioma has not been elucidated. The aim of this study was to 
explore let-7c expression levels and evaluate its function in glioma cells. We first measured the expression 
of let-7c in four glioma cell lines and a normal cell line by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), and the results showed that let-7c was downregulated in glioma cells. By 
applying gain-of-function and loss-of-function assays, the experiments suggested that dysregulation of let-7c 
could obviously affect cell proliferation, metastasis, and invasion. Based on online bioinformatics analysis 
and Dual-Luciferase Reporter assays, we found that E2F5 was a target gene of let-7c and contributed to the 
function of let-7c in glioma cells. Our investigations indicated that loss of let-7c contributed to the progression of glioma cells.},
DOI = {10.3727/096504018X15164123839400}
}