@Article{096504017X14965095236521,
AUTHOR = {Shilei Wang, Yuzuo Hui, Xiaoming Li, Qingbin Jia},
TITLE = {Silencing of lncRNA CCDC26 Restrains the Growth and Migration  of Glioma Cells In Vitro and In Vivo via Targeting miR-203},
JOURNAL = {Oncology Research},
VOLUME = {26},
YEAR = {2018},
NUMBER = {8},
PAGES = {1143--1154},
URL = {http://www.techscience.com/or/v26n8/56729},
ISSN = {1555-3906},
ABSTRACT = {Gliomas are the most common primary brain tumors with high mortality. The treatment for gliomas is largely 
limited due to its uncomprehending pathological mechanism. Here we aimed to investigate the effect of long 
noncoding RNA (lncRNA) coiled-coil domain-containing 26 (CCDC26) in glioma progression. In our study, 
the expression of CCDC26 was found upregulated in glioma tissues and cell lines compared with normal tissues 
and cell lines. Further exploration detected decreased cell proliferation and increased cell apoptosis in U-251 
and M059J cells transfected with CCDC26-siRNA. In addition, the silencing of CCDC26 strongly reduced the 
wound closing rate and the number of invasive cells compared with the scramble group. Simultaneously, the 
expression of miR-203 was found suppressed in glioma tissues and cells lines. Suppressed level of miR-203 
was then elevated in U-251 and M059J cells transfected with CCDC26-siRNA. The result of the luciferase 
activity assay also showed that the luciferase activity was strongly strengthened by adding the miR-203 inhibitor into the CCDC26 WT group. Moreover, CDCC26-siRNA counteracted the effect of the miR-203 inhibitor 
in facilitating cell viability and mobility in U-251 cells. The in vivo experiment also revealed that CCDC26-
siRNA inhibited glioma growth and metastasis. Taken together, our research indicated a CCDC26/miR-203 
pathway in regulating the growth and metastasis of gliomas, providing new viewpoints and promising targets 
for glioma therapy.},
DOI = {10.3727/096504017X14965095236521}
}