@Article{096504017X15041934685237,
AUTHOR = {Jie Shen, Jianhua Zhang, Minhui Xiao, Junfeng Yang, Ningnan Zhang},
TITLE = {miR-203 Suppresses Bladder Cancer Cell Growth and Targets Twist1},
JOURNAL = {Oncology Research},
VOLUME = {26},
YEAR = {2018},
NUMBER = {8},
PAGES = {1155--1165},
URL = {http://www.techscience.com/or/v26n8/56730},
ISSN = {1555-3906},
ABSTRACT = {miR-203 is an epigenetically silenced tumor-suppressive microRNA in tumors. This study was designed to 
investigate the effects of miR-203 on the proliferation, migration, invasion, and apoptosis of bladder cancer 
(BCa) cells. The expression levels of miR-203 in BCa tissues, normal adjacent tissues, and BCa cell lines were 
detected. BCa cells were transfected with miR-203 mimic and inhibitor to investigate the effect of miR-203 on 
cell functions and the epithelial–mesenchymal transition (EMT). Cotransfection with miR-203 inhibitor and 
shRNA of the predicted target gene Twist1 (si-Twist1) was performed to investigate the target relationship of 
miR-203 and Twist1. The effects of knockdown of Twist1 on cell functions were also investigated. The expression of miR-203 was significantly reduced in BCa tissues and cells, in comparison with the control. miR-203 
mimic significantly reduced cell viability, invasion, migration, and EMT, and enhanced cell apoptosis. On the 
contrary, miR-203 inhibitor showed the opposite results. However, the administration of si-Twist1 cancelled 
the effect of miR-203 inhibitor on cell proliferation, apoptosis, invasion, and migration. These demonstrated 
that miR-203 may function as a tumor-suppressive microRNA in BCa by negatively targeting Twist1. Both 
Twist1 and miR-203 might be explored as potential targets for studying the mechanism related to BCa pathogenesis and therapy.},
DOI = {10.3727/096504017X15041934685237}
}