@Article{096504018X15178736525106,
AUTHOR = {Xiaoyun Liu,  Shuguang Li, Yanyan Li, Bo Cheng, Bo Tan, Gang Wang},
TITLE = {Puerarin Inhibits Proliferation and Induces Apoptosis by Upregulation  of miR-16 in Bladder Cancer Cell Line T24},
JOURNAL = {Oncology Research},
VOLUME = {26},
YEAR = {2018},
NUMBER = {8},
PAGES = {1227--1234},
URL = {http://www.techscience.com/or/v26n8/56738},
ISSN = {1555-3906},
ABSTRACT = {Bladder cancer (BC) is a common disease of the urinary system. Puerarin is a flavonoid extracted from 
<i>Pueraria lobata</i>. However, the role of puerarin in BC remains unclear. Hence, this study aimed to investigate 
the effect of puerarin on BC cells. Cell viability, proliferation, and apoptosis were measured by CCK-8, BrdU 
assay, and flow cytometry analysis, respectively. The expressions of miR-16, apoptosis-related factors, and the 
main factors of the NF-kB pathway were analyzed by qRT-PCR and Western blot. In this study, we found that 
cell viability and proliferation were significantly reduced, cell apoptosis was enhanced, and the mRNA level 
of miR-16 was upregulated in puerarin-treated T24 cells. Further, silencing of miR-16 inhibited the decrease 
in cell viability and the increase in apoptosis. The expression of main factors involved in the NF-kB signaling pathway was downregulated in the puerarin group, while miR-16 silencing alleviated these downregulations. More importantly, puerarin deactivated the NF-kB signaling pathway via upregulation of miR-16. Also, 
miR-16 downregulated COX-2 expression via deactivation of the NF-kB signaling pathway. This study demonstrated that puerarin could inhibit cell proliferation, promote cell apoptosis, and deactivate NF-kB signaling 
pathway via upregulation of miR-16 in T24 cells.},
DOI = {10.3727/096504018X15178736525106}
}