@Article{096504018X15179680859017,
AUTHOR = {Xuyan Li, Xuanfang Zhong, Xiuhua Pan, Yan Ji},
TITLE = {Tumor-Suppressive MicroRNA-708 Targets Notch1 to Suppress Cell  Proliferation and Invasion in Gastric Cancer},
JOURNAL = {Oncology Research},
VOLUME = {26},
YEAR = {2018},
NUMBER = {9},
PAGES = {1317--1326},
URL = {http://www.techscience.com/or/v26n9/56761},
ISSN = {1555-3906},
ABSTRACT = {Growing evidence has demonstrated that numerous microRNAs (miRNAs) may participate in the regulation 
of gastric carcinogenesis and progression. This phenomenon suggests that gastric cancer-related miRNAs 
can be identified as effective therapeutic targets for this disease. miRNA-708 (miR-708) has recently been 
reported to be aberrantly expressed in several types of cancer and contribute to carcinogenesis and progression. 
However, the expression level, biological roles, and underlying mechanisms of miR-708 in gastric cancer are 
poorly understood. Here we found that miR-708 was downregulated in gastric cancer tissues and cell lines. 
Downregulated miR-708 expression was significantly associated with lymphatic metastasis, invasive depth, 
and TNM stage. Further investigation indicated that ectopic expression of miR-708 prohibited cell proliferation 
and invasion in gastric cancer. Bioinformatics analysis showed that Notch1 was a potential target of miR-708. 
Notch1 was further confirmed as a direct target gene of miR-708 in gastric cancer by dual-luciferase reporter 
assay, reverse transcription quantitative polymerase chain reaction, and Western blot analysis. Furthermore, an 
inverse association was found between miR-708 and Notch1 mRNA levels in gastric cancer tissues. In addition, restored Notch1 expression rescued the inhibitory effects on gastric cancer cell proliferation and invasion 
induced by miR-708 overexpression. Our findings highlight the tumor-suppressive roles of miR-708 in gastric 
cancer and suggest that miR-708 may be investigated as a novel target for gastric cancer treatment.},
DOI = {10.3727/096504018X15179680859017}
}