@Article{096504018X15188367859402,
AUTHOR = {Chengwei Zhou, Jianxiang Xu, Jinti Lin, Renjin Lin, Kai Chen, Jianzhong Kong, Xiaolong Shui},
TITLE = {Long Noncoding RNA FEZF1-AS1 Promotes Osteosarcoma Progression  by Regulating the miR-4443/NUPR1 Axis},
JOURNAL = {Oncology Research},
VOLUME = {26},
YEAR = {2018},
NUMBER = {9},
PAGES = {1335--1343},
URL = {http://www.techscience.com/or/v26n9/56763},
ISSN = {1555-3906},
ABSTRACT = {Long noncoding RNA (lncRNA) FEZF1-AS1 was demonstrated to facilitate cell proliferation and migration 
in some cancers. However, the functions of FEZF1-AS1 and its molecular mechanism in osteosarcoma remain 
to be elucidated. In our study, we found that the expression of FEZF1-AS1 was upregulated in osteosarcoma 
samples and cell lines compared with normal tissues or cells. Besides, we showed that the expression levels 
of FEZF1-AS1 in osteosarcoma patients were positively correlated with tumor metastasis and TNM stage. 
Additionally, FEZF1-AS1 knockdown inhibited cell proliferation, migration, and invasion in U2OS and MG63 
cells, while upregulation had the opposite effects in vitro. Moreover, FEZF1-AS1 depletion inhibited tumor 
growth and metastasis in vivo. We found that FEZF1-AS1 sponged miR-4443 to promote NUPR1 expression 
in U2OS and MG63 cells. Furthermore, knockdown of miR-4443 abrogated FEZF1-AS1 silencing-induced 
inhibition of cell proliferation, migration, and invasion in osteosarcoma. Finally, we found that restoration of 
NUPR1 rescued the proliferation, migration, and invasion abilities of FEZF1-AS1-depleted U2OS and MG63 
cells. Our study indicated that FEZF1-AS1 could promote osteosarcoma progression by sponging miR-4443 to 
promote NUPR1 expression. The FEZF1-AS1/miR-4443/NUPR1 axis may act as a novel therapeutic strategy 
for osteosarcoma treatment.},
DOI = {10.3727/096504018X15188367859402}
}