@Article{096504018X15178768577951,
AUTHOR = {Lei Chen, Yuhai Wang, Jianqing He, Chunlei Zhang, Junhui Chen, Dongliang Shi},
TITLE = {Long Noncoding RNA H19 Promotes Proliferation and Invasion  in Human Glioma Cells by Downregulating miR-152},
JOURNAL = {Oncology Research},
VOLUME = {26},
YEAR = {2018},
NUMBER = {9},
PAGES = {1419--1428},
URL = {http://www.techscience.com/or/v26n9/56772},
ISSN = {1555-3906},
ABSTRACT = {miR-152 and lncRNA H19 have been frequently implicated in various cellular processes including cell proliferation, invasion, angiogenesis, and apoptosis. However, the interaction between miR-152 and H19 in glioma 
has never been reported. RT-qPCR was used to examine the expression of miR-152 and H19 in human glioma 
cell lines and normal human astrocytes (NHAs). The interaction between miR-152 and lncRNA H19 was 
assessed by dual-luciferase reporter assay. MTT assay and Transwell invasion assay were used to determine 
the proliferation and invasion of U251 and U87 cells. A xenograft tumor experiment was performed to confirm 
the role of H19 in vivo. The results showed that H19 expression was upregulated and miR-152 expression was 
downregulated in human glioma cell lines. H19 downregulation or miR-152 upregulation suppressed glioma 
cell proliferation and invasion in vitro. Moreover, H19 and miR-152 directly regulated each other. Furthermore, 
decreased miR-152 expression alleviated si-H19-induced inhibitory effects on proliferation and invasion in 
glioma cells. As expected, H19 silencing hindered glioma growth in vivo. Taken together, H19 promoted 
glioma cell proliferation and invasion by negatively regulating miR-152 expression, providing evidence for the 
potential application of H19 as a biomarker and therapy target for glioma.},
DOI = {10.3727/096504018X15178768577951}
}