@Article{096504018X15202988139874,
AUTHOR = {Jinfeng Qu, Lei Zhang, Lanyu Li, Yujie Su},
TITLE = {miR-148b Functions as a Tumor Suppressor by Targeting Endoplasmic  Reticulum Metallo Protease 1 in Human Endometrial Cancer Cells},
JOURNAL = {Oncology Research},
VOLUME = {27},
YEAR = {2019},
NUMBER = {1},
PAGES = {81--88},
URL = {http://www.techscience.com/or/v27n1/48663},
ISSN = {1555-3906},
ABSTRACT = {This study investigated the tumor-suppressive role of miR-148b in regulating endoplasmic reticulum metalloprotease 1 (ERMP1) expression and the oxidative stress response in endometrial cancer cells. Human endometrial cancer RL95-2 cells were used and transfected with miR-148b mimic, miR-148b inhibitor, or their 
scrambled negative control. Thereafter, the transfection efficiency was determined by RT-qPCR, and cell proliferation was assessed by MTT assay. The dual-luciferase reporter assay, Western blot, and RT-qPCR were 
conducted to determine the target gene of miR-148b. ERMP1 is a putative target of miR-148b, and thereby the 
overexpression and downregulation of ERMP1 on the proliferation of RL95-2 cells were assessed. Next, the 
expressions of hypoxia-inducible factor 1 (HIF-1) and nuclear factor erythroid 2-related factor 2 (Nrf2) were 
analyzed by Western blot. Intracellular reactive oxygen species (ROS) was determined using dichlorofluorescin diacetate (DCFDA). Results showed that differential expression of miR-148b or ERMP1 was observed in 
normal endometrial tissues and endometrial cancerous tissues. Enhanced expression of miR-148b effectively 
inhibited proliferation of RL95-2 cells. ERMP1 was the target of miR-148b. ERMP1 silencing obviously suppressed proliferation of RL95-2 cells. Thus, miR-148b repressed cell proliferation, likely through downregulating ERMP1. Furthermore, it was observed that miR-148b significantly decreased expression of HIF-1 and 
Nrf2 by downregulating ERMP1. The intracellular ROS level was enhanced by miR-148b via downregulating 
ERMP1. To conclude, our results suggested that miR-148b suppressed cell proliferation and regulated the oxidative stress response in human endometrial cancer RL95-2 cells by inhibiting ERMP1.},
DOI = {10.3727/096504018X15202988139874}
}