@Article{096504018X15195193936573,
AUTHOR = {Xingxiang Liu, Lin Cui, Dong Hua},
TITLE = {Long Noncoding RNA XIST Regulates miR-137–EZH2 Axis to Promote  Tumor Metastasis in Colorectal Cancer},
JOURNAL = {Oncology Research},
VOLUME = {27},
YEAR = {2019},
NUMBER = {1},
PAGES = {99--106},
URL = {http://www.techscience.com/or/v27n1/48665},
ISSN = {1555-3906},
ABSTRACT = {We aimed to investigate the significant role of long noncoding RNA X inactive specific transcript (XIST) 
in regulating tumor metastasis in colorectal cancer (CRC), as well as its possible mechanism. Expression of 
lncRNA XIST in CRC tissues and CRC cells was detected. CRC cells were transfected with pc-XIST, blank 
control si-XIST, or si-control, and then the effects of lncRNA XIST on CRC cell migration and invasion were 
investigated, along with the interaction between lncRNA XIST and miR-137. lncRNA XIST was upregulated 
in CRC tissues. Compared with HT29 cells that had low metastatic potential, XIST was markedly more highly 
expressed in LoVo cells that had a higher metastatic potential. Overexpression of XIST promoted the migratory and invasive potential of HT29 cells, while knockdown of XIST inhibited the migratory and invasive 
potential of LoVo cells. Moreover, epithelial–mesenchymal transition (EMT) markers, including E-cadherin, 
N-cadherin, and vimentin, exhibited corresponding expression changes. In addition, miR-137 was inhibited by 
XIST, and inhibition of miR-137 could reverse the effects of knockdown of XIST on the migratory and invasive 
potential of LoVo cells. Furthermore, enhancer of zeste homolog 2 (EZH2) was confirmed as a target of miR-
137. Our data reveal that lncRNA XIST may promote tumor metastasis in CRC possibly through regulating the 
miR-137–EZH2 axis. lncRNA XIST may serve as a prognostic indicator for CRC progression.},
DOI = {10.3727/096504018X15195193936573}
}