@Article{096504018X15213031799835,
AUTHOR = {Yang Cao, Xu Shi, Yingmin Liu, Ren Xu, Qing Ai},
TITLE = {MicroRNA-338-3p Inhibits Proliferation and Promotes Apoptosis of Multiple  Myeloma Cells Through Targeting Cyclin-Dependent Kinase 4},
JOURNAL = {Oncology Research},
VOLUME = {27},
YEAR = {2019},
NUMBER = {1},
PAGES = {117--124},
URL = {http://www.techscience.com/or/v27n1/48667},
ISSN = {1555-3906},
ABSTRACT = {MicroRNA-338-3p (miR-338-3p) has been reported to be a tumor suppressor in multiple cancer types. 
However, the biological role of miR-338-3p and its underlying mechanism in multiple myeloma (MM) 
remain unclear. In the present study, we investigated the biological role and potential of miR-338-3p in 
MM. We found that miR-338-3p was significantly decreased in newly diagnosed and relapsed MM tissues and cell lines. Overexpression of miR-338-3p in MM cells significantly inhibited proliferation and 
promoted apoptosis, caspase 3, and caspase 8 activity. Bioinformatics algorithm analysis predicted that 
cyclin-dependent kinase 4 (CDK4) was a direct target of miR-338-3p, and this was experimentally verified 
by a dual-luciferase reporter assay. Furthermore, overexpression of miR-338-3p inhibited CDK4 expression 
on mRNA and protein levels. Of note, the restoration of CDK4 expression markedly abolished the effect of 
miR-338-3p overexpression on cell proliferation, apoptosis, caspase 3, and caspase 8 activities in MM cells. 
Taken together, the present study is the first to demonstrate that miR-338-3p functions as a tumor suppressor 
in MM through inhibiting CDK4. This finding implies that miR-338-3p is a potential therapeutic target for 
the treatment of MM.},
DOI = {10.3727/096504018X15213031799835}
}