TY  - EJOU
AU  - Cao, Yang 
AU  - Shi, Xu 
AU  - Liu, Yingmin 
AU  - Xu, Ren 
AU  - Ai, Qing 

TI  - MicroRNA-338-3p Inhibits Proliferation and Promotes Apoptosis of Multiple  Myeloma Cells Through Targeting Cyclin-Dependent Kinase 4
T2  - Oncology Research

PY  - 2019
VL  - 27
IS  - 1
SN  - 1555-3906

AB  - MicroRNA-338-3p (miR-338-3p) has been reported to be a tumor suppressor in multiple cancer types. 
However, the biological role of miR-338-3p and its underlying mechanism in multiple myeloma (MM) 
remain unclear. In the present study, we investigated the biological role and potential of miR-338-3p in 
MM. We found that miR-338-3p was significantly decreased in newly diagnosed and relapsed MM tissues and cell lines. Overexpression of miR-338-3p in MM cells significantly inhibited proliferation and 
promoted apoptosis, caspase 3, and caspase 8 activity. Bioinformatics algorithm analysis predicted that 
cyclin-dependent kinase 4 (CDK4) was a direct target of miR-338-3p, and this was experimentally verified 
by a dual-luciferase reporter assay. Furthermore, overexpression of miR-338-3p inhibited CDK4 expression 
on mRNA and protein levels. Of note, the restoration of CDK4 expression markedly abolished the effect of 
miR-338-3p overexpression on cell proliferation, apoptosis, caspase 3, and caspase 8 activities in MM cells. 
Taken together, the present study is the first to demonstrate that miR-338-3p functions as a tumor suppressor 
in MM through inhibiting CDK4. This finding implies that miR-338-3p is a potential therapeutic target for 
the treatment of MM.
KW  - Multiple myeloma (MM); MicroRNAs; miR-338-3p; CDK4

DO  - 10.3727/096504018X15213031799835