TY  - EJOU
AU  - Dou, Liyan 
AU  - Han, Kaiyu 
AU  - Xiao, Mochao 
AU  - Lv, Fuzhen 

TI  - miR-223-5p Suppresses Tumor Growth and Metastasis in Non-Small  Cell Lung Cancer by Targeting E2F8
T2  - Oncology Research

PY  - 2019
VL  - 27
IS  - 2
SN  - 1555-3906

AB  - miR-223-5p has been demonstrated to regulate the development and progression of various cancers, such 
as hepatocellular carcinoma, breast cancer, and gastric carcinoma. However, the role of miR-223-5p in nonsmall cell lung cancer (NSCLC) requires further investigation. In this study, we found that the expression 
of miR-223-5p was significantly downregulated in NSCLC tissues and cell lines. Moreover, the expression 
level of miR-223-5p is negatively correlated with the malignance of NSCLC. We found that overexpression 
of miR-223-5p remarkably suppressed the proliferation of NSCLC cells in vitro and in vivo. miR-223-5p 
overexpression also led to reduced migration and invasion in NSCLC cells. Mechanistically, we found that 
E2F8, a key transcription factor involved in many kinds of biological processes, was a direct target gene of 
miR-223-5p. Overexpression of miR-223-5p significantly decreased the mRNA and protein levels of E2F8 in 
NSCLC cells. We also showed that restoration of E2F8 rescued the proliferation, migration, and invasion of 
miR-223-5p-overexpressing NSCLC cells. Taken together, our findings demonstrated that miR-223-5p suppressed NSCLC progression through targeting E2F8.
KW  - miR-223-5p; Non-small cell lung cancer (NSCLC); Growth; Metastasis; E2F8

DO  - 10.3727/096504018X15219188894056