@Article{096504018X15220594629967,
AUTHOR = {Hongliang Yang,
 Lei Yan,
 Kai Sun, Xiaodong Sun, Xudong Zhang,§ Kerui Cai, Tiejun Song},
TITLE = {lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell  Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 (<i>GLI2</i>)},
JOURNAL = {Oncology Research},
VOLUME = {27},
YEAR = {2019},
NUMBER = {3},
PAGES = {359--369},
URL = {http://www.techscience.com/or/v27n3/48599},
ISSN = {1555-3906},
ABSTRACT = {This study aimed to explore the effects of lncRNA BCAR4 on the viability and aggressiveness of non-small cell 
lung cancer (NSCLC) cells. qRT-PCR was used to determine the expression of BCAR4 and <i>GLI2</i> downstream 
genes in NSCLC tissues and cell lines. Chromatin isolation by RNA purification (CHIRP) and Western blot 
were employed to measure the expression of the <i>GLI2</i> downstream proteins. Ki-67 expression in nude mice 
tumors was tested by immunohistochemistry. MTT assay, wound healing assay, and Transwell assay were used 
to assess NSCLC cell viability and aggressiveness, respectively. Tumor xenograft was conducted to determine 
the effects of BCAR4 and <i>GLI2</i> on NSCLC tumorigenesis in vivo. The expression of BCAR4 in NSCLC tissues and cells was significantly higher than the normal level. The overexpression of BCAR4 promoted NSCLC 
cell viability, migration, and invasion. The suppression of BCAR4 and <i>GLI2</i> showed the opposite effects. 
The overexpression of BCAR4 led to an increase in the expression of <i>GLI2</i> downstream proteins, while the 
suppression of BCAR4 and <i>GLI2</i> reduced their expression. In a tumor xenograft assay, the tumors in mice of 
the BCAR4 group showed the biggest volume, while those in mice of the si-GLI2 group showed the smallest 
volume. Ki-67 showed much higher levels in the BCAR4 overexpression group but much lower levels in the 
si-GLI2 group. In summary, the cooperative mechanism of lncRNA BCAR4 and <i>GLI2</i> might provide a new 
opportunity for treating NSCLC.},
DOI = {10.3727/096504018X15220594629967}
}