@Article{096504018X15223159811838,
AUTHOR = {Chao Liu, Min Jian, Hong Qi, Wei-Zheng Mao},
TITLE = {MicroRNA 495 Inhibits Proliferation and Metastasis and Promotes Apoptosis  by Targeting Twist1 in Gastric Cancer Cells},
JOURNAL = {Oncology Research},
VOLUME = {27},
YEAR = {2019},
NUMBER = {3},
PAGES = {389--397},
URL = {http://www.techscience.com/or/v27n3/48602},
ISSN = {1555-3906},
ABSTRACT = {Recently, microRNAs (miRNAs) have been reported to participate in multiple biological processes. However, 
the effects of miR-495 on gastric cancer (GC) remain unclear. The purpose of this study was to explore the 
functions of miR-495 in GC cell proliferation, metastasis, and apoptosis. SGC-7901 and BGC-823 cell lines 
were transfected with miR-495 mimic, miR-495 inhibitor, and negative controls (mimic control and inhibitor 
control). The expressions of miR-495, cell viability, migration, apoptosis, and apoptosis-related factors were 
examined by qRT-PCR, trypan blue staining, Transwell, flow cytometry, and Western blot, respectively. 
Simultaneously, key factor expression levels of EMT were detected by qRT-PCR and Western blot. The 
direct target of miR-495 was confirmed by dual-luciferase assay. Additionally, sh-Twist1, pc-Twist1, and 
corresponding controls were transfected into SGC-7901 and BGC-823 cells, and the protein levels of EMTassociated factors were detected by Western blot. miR-495 was downregulated in GC cells. miR-495 expression level was effectively overexpressed or suppressed in SGC-7901 and BGC-823 cells. Overexpression 
of miR-495 significantly decreased cell viability and migration, increased apoptosis, and inhibited the EMT 
process. Suppression of miR-495 showed contrary results. Twist1 was clarified as a target gene of miR-495, 
and Twist1 silencing obviously reduced the promoting effect of miR-495 suppression on these biological 
processes. Twist1 silencing significantly blocked the EMT process in both SGC-7901 and BGC-823 cells. 
miR-495 inhibited proliferation and metastasis and promoted apoptosis by targeting Twist1 in GC cells. 
These data indicated that miR-495 might be a novel antitumor factor of GC and provide a new method for 
the treatment of GC.},
DOI = {10.3727/096504018X15223159811838}
}