@Article{096504018X15179675206495,
AUTHOR = {Xiaofei Ning, Cong Wang, Meng Zhang, Kecheng Wang},
TITLE = {Ectopic Expression of miR-147 Inhibits Stem Cell Marker  and Epithelial–Mesenchymal Transition (EMT)-Related  Protein Expression in Colon Cancer Cells},
JOURNAL = {Oncology Research},
VOLUME = {27},
YEAR = {2019},
NUMBER = {4},
PAGES = {399--406},
URL = {http://www.techscience.com/or/v27n4/48552},
ISSN = {1555-3906},
ABSTRACT = {Colon cancer is one of the most common cancers in the world. Epithelial-to-mesenchymal transition (EMT) 
is a crucial step in tumor progression and is also involved in the acquisition of stem cell-like properties. Some 
miRNAs have been shown to function as either tumor suppressors or oncogenes in colon cancer. Here we 
investigated the role of miR-147 in the regulation of the stem cell-like traits of colon cancer cells. We observed 
that miR-147 was downregulated in several colon cancer cell lines, and overexpressed miR-147 decreased 
the expression of cancer stem cell (CSC) markers OCT4, SOX2, and NANOG in the colon cancer cell lines 
HCT116 and SW480. Overexpressed miR-147 inhibited EMT by increasing the expression of epithelial 
markers E-cadherin and -catenin while decreasing the expression of mesenchymal markers fibronectin and 
vimentin. Moreover, activation of EMT by TGF- 1 treatment significantly counteracted the inhibitive effect of 
miR-147 on the expression of CSC markers OCT4, SOX2, and NANOG, supporting the idea that overexpressing miR-147 inhibited stem cell-like traits by suppressing EMT in colon cancer. In addition, we found that 
overexpressed miR-147 downregulated the expression of -catenin, c-myc, and survivin, which were related to 
the Wnt/ -catenin pathway. Moreover, treatment of miR-147 mimic-transfected cells with the Wnt/ -catenin 
pathway activator LiCl attenuated the inhibitive effect of the miR-147 mimic on the EMT and stem cell-like 
traits of colon cancer cells, indicating that ectopic expression of miR-147 inhibited stem cell-like traits in colon 
cancer cells by suppressing EMT via the Wnt/ -catenin pathway. In summary, our present study highlighted 
the crucial role of miR-147 in the inhibition of the stem cell-like traits of colon cancer cells and indicated that 
miR-147 could be a promising therapeutic target for colon cancer treatment.},
DOI = {10.3727/096504018X15179675206495}
}