TY  - EJOU
AU  - Gu, Chijiang 
AU  - Zhang, Mingyuan 
AU  - Sun, Weiliang 
AU  - Dong, Changzheng 

TI  - Upregulation of miR-324-5p Inhibits Proliferation and Invasion of Colorectal  Cancer Cells by Targeting ELAVL1
T2  - Oncology Research

PY  - 2019
VL  - 27
IS  - 5
SN  - 1555-3906

AB  - Colorectal cancer (CRC) is a common clinical cancer that remains incurable in most cases. miRNAs are 
reported to play a part in the development of various tumors. In the present study, we found that miR-324-5p 
was downregulated in CRC cells, while ELAV (embryonic lethal, abnormal vision, <i>Drosophila</i>)-like protein 
1 (ELAVL1) showed a higher expression. miR-324-5p transfection significantly inhibited the proliferation 
as well as invasion in both SW620 and SW480 cells. miR-324-5p mimic transfection markedly decreased 
the expression of ELAVL1. Luciferase reporter gene assay confirmed that ELAVL1 is a direct target of miR-
324-5p. Furthermore, cancer invasion factors uPA, uPAR, and MMP-9 were found to drop significantly in 
miR-324-5p-transfected groups. To conclude, our findings indicate that miR-324-5p may play a suppressive 
role in colorectal cell viability and invasion, at least in part, through directly targeting ELAVL1. Therefore, 
miR-234-5p might function as a promising candidate for CRC treatment and deserves deeper research.
KW  - Colorectal cancer (CRC); miR-324-5p; Proliferation; Invasion;  (Embryonic lethal
KW  -  abnormal vision
KW  -  <i>Drosophila</i>)-like protein 1 (ELAVL1)

DO  - 10.3727/096504018X15166183598572