@Article{096504018X15360541345000,
AUTHOR = {Tengyu Li, Jing Zhu, Shuai Zuo, Shanwen Chen, Ju Ma, Yongchen Ma, Shihao Guo, 
Pengyuan Wang, Yucun Liu},
TITLE = {1,25(OH)2D3 Attenuates IL-1b-Induced Epithelial-to-Mesenchymal  Transition Through Inhibiting the Expression of lncTCF7},
JOURNAL = {Oncology Research},
VOLUME = {27},
YEAR = {2019},
NUMBER = {7},
PAGES = {739--750},
URL = {http://www.techscience.com/or/v27n7/48603},
ISSN = {1555-3906},
ABSTRACT = {The activated form of vitamin D3, 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], regulates numerous cellular 
processes, including inhibition of cancer progression. IL-1 has been reported to facilitate cancer development, especially by inducing an epithelial-to-mesenchymal transition (EMT) in several malignant tumors. 
However, the underlying mechanism of 1,25(OH)2D3 and IL-1 in colorectal cancer (CRC) still remains 
largely unknown. To fill in this knowledge gap, we measured cell proliferation and invasion by CCK-8 and 
Transwell assays after stimulation with 1,25(OH)2D3 and IL-1 . E-cadherin and vimentin were chosen as 
markers of EMT measured by immunofluorescence, quantitative real-time PCR (qRT-PCR), and Western 
blot. The expression and function of the vitamin D receptor (VDR) was evaluated by Western blot and 
luciferase reporter assay. qRT-PCR and RNA-FISH were performed to detect the expression and location 
of lncTCF7 in vitro. The binding sites of VDR in the lncTCF7 promoter were confirmed by a chromatin 
immunoprecipitation assay. Based on the above experiments, we found that 1,25(OH)2D3 attenuates IL-1 -
induced increased proliferation and invasion in colorectal cancer through enhancing VDR, which inhibits the 
expression of lncTCF7 by directly binding to its promoter region.},
DOI = {10.3727/096504018X15360541345000}
}