@Article{096504021X16401852341873,
AUTHOR = {Fangjin Lu, Bin Mu, Ge Jin, Lin Zhu, Ping Mu},
TITLE = {MYCN Directly Targets NeuroD1 to Promote Cellular Proliferation  in Neuroblastoma},
JOURNAL = {Oncology Research},
VOLUME = {29},
YEAR = {2021},
NUMBER = {1},
PAGES = {1--10},
URL = {http://www.techscience.com/or/v29n1/48448},
ISSN = {1555-3906},
ABSTRACT = {NeuroD1 is a neuronal differentiation factor that contains a basic helix–loop–helix (bHLH) motif. Recently, 
NeuroD1 was found to be associated with tumorigenesis in neuroblastoma (NB) and is known to promote cell 
proliferation and migration in these cells. Here we found that MYCN regulates the expression of NeuroD1 in 
NB cells and that the downregulation of MYCN using short hairpin RNAs (shRNA) results in the inhibition 
of cellular proliferation in NB cells. Moreover, the phenotype induced by MYCN shRNA was rescued by 
the exogenous expression of NeuroD1. Chromatin immunoprecipitation (ChIP) assay showed that MYCN 
directly binds to the E-box element in the NeuroD1 promoter region. In addition, our evaluation of two clinical databases showed that there was a positive correlation between the expression of MYCN and NeuroD1 in 
NB patients, which supports our in vitro data. In conclusion, this study demonstrates that MYCN-regulated 
NeuroD1 expression is one of the important mechanisms underlying enhanced cellular proliferation induced 
by the increase in MYCN expression in NB, and our results provide an important therapeutic target for NB in 
the future.},
DOI = {10.3727/096504021X16401852341873}
}