@Article{or.2022.03178,
AUTHOR = {JIALIN WU, ZEHONG CHEN, WENWEI LIU, YONGXIN ZHANG, WEI FENG, YUJIE YUAN, JINNING YE, LIANG WANG, SHIRONG CAI, YULONG HE, SUIJING WU, WU SONG},
TITLE = {MicroRNA-188-5p targeting Forkhead Box L1 promotes colorectal cancer progression via activating Wnt/β-catenin signaling},
JOURNAL = {Oncology Research},
VOLUME = {29},
YEAR = {2021},
NUMBER = {2},
PAGES = {119--128},
URL = {http://www.techscience.com/or/v29n2/48801},
ISSN = {1555-3906},
ABSTRACT = {<b>Objective:</b> MicroRNA-188-5p (miR-188) enhances oncologic progression in various human malignancies. This
study aimed to explore its role in colorectal cancer (CRC). <b>Materials and Methods: </b>Human CRC tissues paired with
normal tissues, and several CRC cell lines were utilized. Real-time quantitative PCR was applied to measure the
expression of miR-188. Overexpression and knockdown were used to access the function of miR-188 and to
investigate whether FOXL1/Wnt signaling mediates such function. The proliferation, migration and invasion of cancer
cells were evaluated by CCK8, wound-healing and transwell assays, respectively. Whether FOXL1 acted as a direct
target of miR-188 was verified by dual-luciferase reporter assays. <b>Results: </b>Levels of miR-188 were upregulated in CRC
tissues than in paired-normal tissues, as well as in various CRC cell lines. High expression of miR-188 was strongly
associated with advanced tumor stage, accompanied with significant tumor cell proliferation, invasion and migration.
It was confirmed that FOXL1 played positive crosstalk between miR-188 regulation and downstream Wnt/β-catenin
signaling activation. <b>Conclusions:</b> All findings indicate that miR-188 promotes CRC cell proliferation and invasion
through targeting FOXL1/Wnt signaling and could be served as a potential therapeutic target for human CRC in the
future.},
DOI = {10.32604/or.2022.03178}
}