@Article{or.2022.027904,
AUTHOR = {SHAHRAM NEKOEIAN, TAHEREH ROSTAMI, AMIR NOROUZY, SAFIN HUSSEIN, GHOLAMREZA TAVOOSIDANA, BAHRAM CHAHARDOULI, SHAHRBANO ROSTAMI, YAZDAN ASGARI, ZAHRA AZIZI},
TITLE = {Identification of lncRNAs associated with the progression of acute lymphoblastic leukemia using a competing endogenous RNAs network},
JOURNAL = {Oncology Research},
VOLUME = {30},
YEAR = {2022},
NUMBER = {6},
PAGES = {259--268},
URL = {http://www.techscience.com/or/v30n6/51594},
ISSN = {1555-3906},
ABSTRACT = {Acute lymphoblastic leukemia (ALL) is a malignancy of bone marrow lymphoid precursors. Despite effective
treatments, the causes of its progression or recurrence are still unknown. Finding prognostic biomarkers is needed for
early diagnosis and more effective treatment. This study was performed to identify long non-coding RNAs (lncRNAs)
involved in ALL progression by constructing a competitive endogenous RNA (ceRNA) network. These lncRNAs may
serve as potential new biomarkers in the development of ALL. The GSE67684 dataset identified changes in lncRNAs
and mRNAs involved in ALL progression. Data from this study were re-analyzed, and probes related to lncRNAs
were retrieved. Targetscan, miRTarBase, and miRcode databases were used to identify microRNAs (miRNAs) related
to the discovered genes and lncRNAs. The ceRNA network was constructed, and the candidate lncRNAs were
selected. Finally, the results were validated with reverse transcription quantitative real-time PCR (RT-qPCR). The
ceRNA network outcomes demonstrated that the top lncRNAs associated with altered mRNAs in ALL are IRF1-AS1,
MCM3AP-AS1, TRAF3IP2-AS1, HOTAIRM1, CRNDE, and TUG1. Investigations of the subnets linked to
MCM3AP-AS1, TRAF3IP2-AS1, and IRF1-AS1 indicated that these lncRNAs were considerably related to pathways
associated with inflammation, metastasis, and proliferation. Higher expression levels of IRF1-AS1, MCM3AP-AS1,
TRAF3IP2-AS1, CRNDE, and TUG1 were found in ALL samples compared to controls. The expression of MCM3APAS1, TRAF3IP2-AS1, and IRF1-AS1 is significantly elevated during the progression of ALL, playing an oncogenic
role. Due to their role in the main cancer pathways, lncRNAs could be suitable therapeutic and diagnostic targets in ALL.},
DOI = {10.32604/or.2022.027904}
}