@Article{or.2022.028168,
AUTHOR = {GANG WANG, MINGPING QIAN, WEI JIAN, JUHANG CHU, YIXIANG HUANG},
TITLE = {Has_circ_0000069 expression in breast cancer and its influences on prognosis and cellular activities},
JOURNAL = {Oncology Research},
VOLUME = {31},
YEAR = {2023},
NUMBER = {1},
PAGES = {63--70},
URL = {http://www.techscience.com/or/v31n1/51754},
ISSN = {1555-3906},
ABSTRACT = {Circular RNA (circRNA), as a newly discovered non-coding RNA with important regulatory potential, is closely
related to the occurrence and progression of various tumors. This study aimed to investigate has_circ_0000069 expression
in breast cancer and its influence on cellular activities. Using real-time quantitative polymerase chain reaction,
has_circ_0000069 levels were measured in 137 pairs of tissue specimens, as well as cancer cell lines. The cellular
activities of cell lines were determined by cell counting kit-8 (CCK-8) and Transwell assays. The potential targeting
miRNAs were predicted and verified using an online database and dual-luciferase reporter assay. Has_circ_0000069
was highly expressed in breast cancer tissues and cells. The expression of has_circ_0000069 was associated with the
five-year overall survival of patients. After silencing has_circ_0000069 in breast cancer cells, its expression reduced,
and the ability of cell proliferation, migration, and invasion decreased. MiR-432 was verified as a targeting miRNA of
has_circ_0000069. Has_circ_0000069 expression increased in breast cancer and was negatively related to patient’s
prognosis. Has_circ_0000069 may facilitate breast cancer tumor progression by sponging miR-432. These findings
revealed that has_circ_0000069 may be a biomarker for predicting prognosis and a therapeutic target for treating
patients with breast cancer.},
DOI = {10.32604/or.2022.028168}
}