@Article{or.2025.069781,
AUTHOR = {Rebecca H. Maggs, Marcus J. Brookes, Kenneth S. Rankin},
TITLE = {An <i>In Vitro</i> Investigation of 5-Aminolevulinic Acid Mediated Photodynamic Therapy in Bone Sarcoma},
JOURNAL = {Oncology Research},
VOLUME = {34},
YEAR = {2026},
NUMBER = {2},
PAGES = {0--0},
URL = {http://www.techscience.com/or/v34n2/65573},
ISSN = {1555-3906},
ABSTRACT = { <b>Background:</b> Photodynamic therapy (PDT) may eradicate residual malignant cells following sarcoma resection, through reactive oxygen species (ROS) mediated cytotoxicity, thus improve clinical outcomes. This study aims to assess the efficacy of 5-aminolevulinic acid (5-ALA) as a photosensitizer in combination with red light (RL) for PDT of bone sarcoma cells <i>in vitro</i>. <b>Methods:</b> Three bone sarcoma cell lines underwent treatment with 5-ALA and RL or sham-RL (SL). 5-ALA uptake was assessed using flow cytometry. Production of ROS was measured using CellROX Green staining and fluorescence microscopy. Cell viability was assessed using Cell Counting Kit-8 assays. <b>Results:</b> All cell lines showed significant 5-ALA uptake in comparison to the 0 mM control (<i>p</i> < 0.05). Production of ROS was significantly increased in cells treated with 5-ALA and RL, compared to those treated with RL and no 5-ALA or SL (<i>p</i> < 0.05). Viability was significantly reduced in cells treated with 5-ALA and RL, compared to SL (<i>p</i> < 0.05). At 72 h post-treatment, cell viability ranged from 6%–12% in 0.5 mM 5-ALA and RL-treated cells vs. 90%–137% in 0.5 mM 5-ALA and SL-treated cells. <b>Conclusion:</b> 5-ALA-based PDT led to the desired increased production of ROS and reduction in cell viability in all cell lines. These preliminary <i>in vitro</i> results warrant further study with multicellular spheroid or animal models and suggest PDT has potential to be used as an adjuvant therapy to surgical resection in sarcoma management.},
DOI = {10.32604/or.2025.069781}
}