@Article{or.2026.068508,
AUTHOR = {Min Dong, Zongchang Song, Xiaohui Lu, Minxue Lu, Chen Zhong},
TITLE = {<i>CENPF</i> Promotes Gastric Cancer Proliferation through c-Myc-Mediated GLS1 Upregulation and Glutamine Metabolism},
JOURNAL = {Oncology Research},
VOLUME = {34},
YEAR = {2026},
NUMBER = {3},
PAGES = {0--0},
URL = {http://www.techscience.com/or/v34n3/66255},
ISSN = {1555-3906},
ABSTRACT = { <b>Background:</b> Gastric cancer (GC) remains highly lethal, with metabolic reprogramming as a key hallmark. This study explores Centromere Protein F (CENPF)’s role in GC pathogenesis, specifically its regulation of glutamine metabolism. <b>Methods:</b> The Cancer Genome Atlas–Stomach Adenocarcinoma (TCGA-STAD), GSE19826, and GSE27342 datasets were analyzed by bioinformatics to identify key candidate genes in GC. The function of <i>CENPF</i> was assessed by flow cytometry, colony formation assays, and Cell Counting Kit-8 (CCK-8). RNA sequencing, metabolic profiling, chromatin immunoprecipitation (ChIP), western blot (WB), and luciferase reporter assay were employed to investigate the fundamental mechanisms. <b>Results:</b> <i>CENPF</i> was upregulated in GC tumor samples and had a high diagnostic potential. <i>CENPF</i> knockdown declined cell proliferation, caused G2 arrest, and promoted apoptosis in GC cells. RNA sequencing revealed that <i>CENPF</i> was involved in glutamine metabolism. <i>CENPF</i> overexpression enhanced glutamine consumption and glutamate production, while glutamine deficiency reversed <i>CENPF</i>-mediated cell survival. CENPF stabilized cellular myelocytomatosis (c-Myc) by preventing proteasomal degradation, bound to the glutaminase (GLS) promoter, promoting glutamine metabolism. Overexpression of GLS or <i>c-Myc</i> rescued the <i>CENPF</i> knockdown’s inhibitory effect on GC cell growth. <b>Conclusion:</b> Our findings identify a new <i>CENPF</i>/<i>c-Myc</i>/<i>GLS</i> axis that affects glutamine metabolism and cell survival in GC, implying that <i>CENPF</i> might be a novel target for the treatment of GC.},
DOI = {10.32604/or.2026.068508}
}