TY  - EJOU
AU  - Li, Jiaxi 
AU  - Iyer, Deepak 
AU  - Sui, Siming 
AU  - Huang, Zheng 
AU  - Sin, Ryan Wai-Yan 
AU  - Man, Abraham Tak-Ka 
AU  - Law, Wai-Lun 
AU  - Foo, Chi-Chung 
AU  - Ng, Lui 

TI  - piR-37524 Overexpression in Colorectal Cancer: A Potential Diagnostic Bio-Marker and Therapeutic Target
T2  - Oncology Research

PY  - 2026
VL  - 34
IS  - 4
SN  - 1555-3906

AB  -  <b>Objectives:</b> Piwi-associated RNAs are small non-coding RNAs implicated in cancer, yet few have been characterized in colorectal cancer (CRC). This study aimed to identify a CRC-related piRNA and investigate its clinical relevance, biological function, and biomarker potential. <b>Methods:</b> Candidates were identified by reanalysis of small-RNA sequencing. piR-37524 was quantified by quantitative real-time polymerase chain reaction (qRT-PCR) in colorectal cancer tissues, matched adjacent non-tumor tissues, colorectal adenomas, liver metastases, and serum samples from patients and healthy controls. Clinicopathological correlations and diagnostic performance were evaluated. Functional assays included 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) proliferation, colony formation, and wound-healing migration in HCT116 and HT29 cells after piR-37524 inhibition. RNA sequencing and Western blotting examined epithelial–mesenchymal transition (EMT) markers and nuclear factor-kappa B (NF-κB) components. <b>Results:</b> piR-37524 was significantly overexpressed in CRC compared with adjacent non-tumor tissues and was associated with larger tumor size, poorer differentiation, and distant metastasis. Elevated expression was also observed in colorectal adenomas and liver metastases. Serum piR-37524 levels were increased in patients with adenomas and CRC compared with healthy controls, indicating diagnostic potential. Functional assays demonstrated that piR-37524 inhibition suppressed CRC cell proliferation and migration, accompanied by changes consistent with epithelial–mesenchymal transition regulation. Mechanistic analyses implicated tumor necrosis factor alpha-induced protein 3 (TNFAIP3)-associated NF-κB signaling. <b>Conclusions:</b> piR-37524 is an oncogenic piRNA in CRC that promotes progression via the TNFAIP3/NF-κB/EMT axis, serving as a potential pan-stage diagnostic biomarker and therapeutic target.
KW  - Colorectal cancer; piRNAs; tumor biomarker

DO  - 10.32604/or.2026.074981