TY  - EJOU
AU  - Yang, Tingting 
AU  - Guan, Meng 
AU  - Guan, Xin 
AU  - Kang, Lihua 
AU  - Wang, Xiaomeng 
AU  - Guan, Yanjie 
AU  - Yang,  
AU  - Deng, Wei 
AU  - Wang, Guoxiang 

TI  - Breast Cancer Cell-Derived Exosomal miR-92b-3p Promotes Tumor Angiogenesis and Metastasis by Suppressing PTEN in Vascular Endothelial Cells
T2  - Oncology Research

PY  - 2026
VL  - 34
IS  - 8
SN  - 1555-3906

AB  - <b>Background:</b> Tumor-driven vascular remodeling is crucial for breast cancer metastasis; yet, the role of tumor-derived exosomal miRNAs in this process remains underexplored. This study aimed to investigate the clinical relevance and the underlying mechanism of breast cancer-derived exosomal miR-92b-3p in endothelial reprogramming. <b>Methods:</b> miR-92b-3p expression was evaluated in the TCGA cohort and clinical patient samples. The effects of exosomal miR-92b-3p from breast cancer cells on recipient human microvascular endothelial cells (HMVECs) were assessed using <i>in vitro</i> angiogenesis, migration, and permeability assays, alongside <i>in vivo</i> murine xenograft models. Mechanistic targets were validated via dual-luciferase and rescue experiments. <b>Results:</b> miR-92b-3p was significantly upregulated in breast cancer tissues and plasma exosomes, correlating with advanced stages, poor survival, and exhibiting high diagnostic accuracy. Breast cancer-derived exosomes effectively transferred miR-92b-3p into HMVECs, significantly promoting angiogenesis, endothelial migration, and transendothelial permeability. <i>In vivo</i>, overexpression of miR-92b-3p accelerated tumor growth, vascularization, and circulating tumor cell (CTC) dissemination. Mechanistically, exosomal miR-92b-3p directly targeted and suppressed PTEN in endothelial cells; moreover, restoring PTEN expression fully abrogated the exosome-induced pro-angiogenic and hyperpermeable phenotypes. <b>Conclusions:</b> Breast cancer-derived exosomal miR-92b-3p disrupts the vascular barrier and promotes tumor angiogenesis by targeting endothelial PTEN, thereby facilitating metastasis. Circulating exosomal miR-92b-3p represents a promising candidate liquid-biopsy biomarker for breast cancer progression.
KW  - Breast cancer; exosomes; miR-92b-3p; angiogenesis; vascular permeability

DO  - 10.32604/or.2026.083563