Open Access
ARTICLE
Comparative Non Participating Transcriptome Analysis Response to Low Phosphorus by CmPht1;2 in Chrysanthemum
Chen Liu1,2 , Xiaowei Tang1,2, Xiaohui Qu1,2 , Fadi Chen1, Jiafu Jiang1 and Sumei Chen1,*
1
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University,
Key Laboratory of Flower Biology and Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology
of Ornamental Plants in East China, National Forestry and Grassland Administration, Nanjing, 210095, China
2
Nanjing Institute of Agricultural Sciences, Jiangsu Academy of Agricultural Sciences, Nanjing, 210046, China
* Corresponding Author: Sumei Chen. Email:
(This article belongs to this Special Issue: Identification of Genetic/Epigenetic Components Responding to Biotic and Abiotic Stresses in Crops)
Phyton-International Journal of Experimental Botany 2023, 92(5), 1391-1404. https://doi.org/10.32604/phyton.2023.027231
Received 20 October 2022; Accepted 28 November 2022; Issue published 09 March 2023
Abstract
Chrysanthemum morifolium Ramat is one of the four major cut flowers in the world. Pht1 family is focus on the
uptake and transport of phosphate in plants. In our previous studies,
CmPht1;2 overexpression line (Oe1) had
higher phosphate contents both in roots and shoots, and its root development was significantly enhanced than
wild type (WT) at low phosphorus conditions in chrysanthemum. Metabolomics analysis showed that several
metabolites had a change in pyruvate metabolism and tricarboxylic acid (TCA) cycle pathway. To explore the
gene difference expression and the change of metabolic pathway between
CmPht1;2-Oe1 and WT, we conducted
the transcriptome analysis. A total of 617,681 and 207,271 unigenes were obtained from roots and shoots, respectively. They were classified into biological process, cellular component and molecular function by Gene Ontology
(GO). In addition, 450 different expression genes (DEGs) were found in the roots after 2 d treatment, and
1,787 DEGs were identified in shoots after 7 d treatment under LP condition between Oe1 and WT. From the
top 20 pathways of DEGs assigned by Kyoto Encyclopedia of Genes and Genomes (KEGG), TCA cycle and pyruvate metabolism pathways mostly affected by overexpression of
CmPht1;2 attracted our attention. This research
will be helpful for elucidating the mechanism of effects by
CmPht1;2 overexpression on growth, development and
stress tolerance.
Keywords
Supplementary Material
Supplementary Material File
Cite This Article
Liu, C., Qu, X., Jiang, J. (2023). Comparative Non Participating Transcriptome Analysis Response to Low Phosphorus by
CmPht1;2 in Chrysanthemum.
Phyton-International Journal of Experimental Botany, 92(5), 1391–1404.