@Article{phyton.2026.083644,
AUTHOR = {Siyi Fang, Bin Zheng, Jiao Ma, Shengwen Tang, Lanying Chen, Qiumei Quan},
TITLE = {Optimization of Culture Medium and Conditions for <i>In Vitro</i> Pollen Germination in <i>Hibiscus mutabilis</i> Linn.},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {},
YEAR = {},
NUMBER = {},
PAGES = {{pages}},
URL = {http://www.techscience.com/phyton/online/detail/27226},
ISSN = {1851-5657},
ABSTRACT = {Pollen viability plays a pivotal role in determining fertilization success, directly influencing fruit and seed production, as well as the genetic improvement and conservation of flowering plants. This study sought to establish the optimal culture medium and environmental conditions for <i>in vitro</i> pollen germination and pollen tube growth in <i>Hibiscus mutabilis</i> Linn., a species of notable ornamental and ecological significance. We employed Brewbaker and Kwack medium(BK) supplemented with varying concentrations of sucrose, H<sub>3</sub>BO<sub>3</sub>, CaCl<sub>2</sub>, MgSO<sub>4</sub>·7H<sub>2</sub>O, and KNO<sub>2</sub> to systematically evaluate the effects of these components on pollen viability. Additionally, we investigated the impact of environmental factors including temperature, light intensity, humidity, and pH. The optimal culture medium consisted of 300 g/L sucrose, 50 mg/L H<sub>3</sub>BO<sub>3</sub>, and 150 mg/L CaCl<sub>2</sub>, yielding maximum germination rates (69.3% for red pollen, 65.7% for white pollen) and tube lengths (467.5 μm and 428.6 μm, respectively). Temperature was identified as a crucial factor, with 20°C being optimal. High humidity (100%) and slightly acidic pH (6.1) further enhanced pollen viability. Pollen germination was most successful under dark conditions, while moderate light intensity (6000–12,000 lux) facilitated optimal pollen tube elongation. This study provides a systematic investigation of pollen germination in <i>H. mutabilis</i>, offering critical insights for hybrid breeding programs and conservation efforts.},
DOI = {10.32604/phyton.2026.083644}
}