TY  - EJOU
AU  - Pang, Chunmei 
AU  - Sui, Bo 
AU  - Wang, Kangyu 

TI  - Comprehensive Genome Identification Analysis of <i>EXO70</i> Gene Family in <i>Panax ginseng</i> and <i>PgEXO70</i> Gene Expression under Methyl Jasmonate Regulation
T2  - Phyton-International Journal of Experimental Botany

PY  - 
VL  - 
IS  - 
SN  - 1851-5657

AB  - As an important medicinal plant in China, <i>Panax ginseng</i> is rich in secondary metabolites and is widely used in immune regulation, antitumor, anti-aging applications. The exocyst complex is a crucial protein complex that regulates vesicle trafficking in plants and plays key roles in cellular secretion, polar growth, and stress responses. Methyl jasmonate (MeJA) is a widely occurring derivative of jasmonic acid (JA) in plants and belongs to the category of endogenous signaling molecules in plants, which significantly regulates plant defense and secondary metabolism. MeJA is a key signaling molecule that significantly regulates plant defense and secondary metabolic pathways and has been shown to induce ginsenoside synthesis in <i>P. ginseng</i>. Despite the absence of reports on the effects of the <i>EXO70</i> gene family in ginseng under MeJA treatment, this study comprehensively screened, identified, and systematically analyzed the <i>EXO70</i> gene family in ginseng. Additionally, <i>PgEXO70</i> candidate genes that exhibited significant responses to MeJA were identified, and an extensive investigation of <i>PgEXO70</i> gene expression patterns was conducted. Utilizing ginseng genome and transcriptome data, we identified 58 members of the <i>EXO70</i> gene family in ginseng. We conducted the analysis of their phylogenetic relationships, gene structures, chromosomal distributions, cis-regulatory elements, co-expression network, and gene expression patterns. In addition, we treated ginseng adventitious roots with MeJA and investigated the expression trends of the four <i>PgEXO70</i> genes containing the highest number of MeJA elements. All four genes responded to MeJA treatment, but the expression of <i>PgEXO70-18</i> was significantly elevated at all treatment times. These results provide the genetic resource support for further exploration of the functions of <i>EXO70</i> genes in <i>P. ginseng</i> and their roles in plant hormone regulation and secondary metabolism.
KW  - <i>Panax ginseng</i>; exocyst complex; <i>EXO70</i> gene family; methyl jasmonate regulation; gene expression pattern

DO  - 10.32604/phyton.2026.085307