TY  - EJOU
AU  - Tuzin, Liliia 
AU  - Grytsyk, Andriy 
AU  - Raal, Ain 
AU  - Hrytsyk, Roman 
AU  - Jakštas, Valdas 
AU  - Žvikas, Vaidotas 
AU  - Dubel, Nataliia 
AU  - Koshovyi, Oleh 

TI  - Phytochemical Profiling and Biological Activity Screening of <i>Anemone nemorosa</i> L. Extracts with Anti-Inflammatory and Hepatoprotective Effects
T2  - Phyton-International Journal of Experimental Botany

PY  - 
VL  - 
IS  - 
SN  - 1851-5657

AB  - This study represents a preliminary phytochemical and pharmacological screening of <i>Anemone nemorosa</i> L. collected in Ukraine. The aim was to characterize the phytochemical composition of the herb, rhizomes, and extracts obtained using solvents of different polarity, and to perform an initial evaluation of their toxicity, anti-inflammatory, hemostatic, and hepatoprotective activities. UPLC–MS/MS analysis identified 12 phenolic compounds, including flavonoids and hydroxycinnamic acids, with the highest level observed in AN-40 extract (obtained using 40% ethanol). Their content varies depending on the plant organ, location, and year of collection. For the first time, amino acid profiling identified 14 amino acids, complementing the phytochemical characterization of <i>A. nemorosa</i> herb and its extracts. The composition of phenolic compounds and amino acids depended on the extraction solvent. The highest total phenolic content was observed in AN-40 extract, whereas the highest flavonoid content was obtained with 70% ethanol. Toxicological evaluation demonstrated that <i>A. nemorosa</i> extracts may be classified as low-toxic substances (toxicity class IV), with no mortality observed at a dose of 5000 mg/kg. Preliminary pharmacological screening using single-dose evaluations in established experimental models indicated anti-inflammatory and hepatoprotective effects of <i>A. nemorosa</i> extracts. The extracts exhibited only weak hemostatic activity. Among the investigated extracts, AN-40 showed the most pronounced anti-inflammatory and hepatoprotective activities under the experimental conditions used. These preliminary findings warrant further dose–response, mechanistic, and bioactivity-guided studies.
KW  - <i>Anemone nemorosa</i>; wood anemone; herb; extract; phenolic substances; hepatoprotective activity

DO  - 10.32604/phyton.2026.089037