@Article{phyton.2006.75.109,
AUTHOR = {Ojeda-Zacarías  Ma del Carmen, Hugo A  Luna-Olvera, Lilia H  Morales-Ramos, María J  Verde-Star, Teresa E  Torres-Cepeda, Benito  Pereyra-Alférez, Leobardo  Iracheta-Donjuan, Emilio  Olivares-Sáenz, Raúl  Salazar-Sáenz, Elizabeth  Cárdenas-Cerda},
TITLE = {<i>In vitro</i> multiplication of the Piñón Azul Pinus maximartinezii (Rzedowski)},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {75},
YEAR = {2006},
NUMBER = {all},
PAGES = {109--113},
URL = {http://www.techscience.com/phyton/v75nall/36867},
ISSN = {1851-5657},
ABSTRACT = {<i>Pinus maximartinezii</i> (Rzedowski) is an endemic endangered species, confined to a single population of approximately 2000 to 2500 mature trees. It covers about 400 ha in southern Zacatecas, Mexico. The success of tissue culture techniques for germplasm preservation depends on regeneration of cultures. The objective of this study was to achieve an <i>in vitro</i> proliferation protocol using organogenesis technique for <i>Pinus maximartinezii</i>. Mature seeds were surface sterilized in 6% H2O2 v/v. Isolated cotyledons and zygotic embryos were cultured on shoot induction media. DCR and GD media were supplemented with 0.3 and 0.5 mgl-1 BAP; 0.01 mgl-1 ANA and vitamin solution. Explants were incubated at 26 ± 2ºC under a 16h photoperiod. The explants were transferred every 15 days to hormone-free medium (DCR and GD) for a period of 6 wk for bud development. The number of explants forming buds was determined. After induction of buds, the explants were transferred to a hormone-free basal medium, to which 0.1% activated charcoal was added. After 8 wk, the number of shoots per embryo was evaluated. Effects of either basal media or plant growth regulator concentrations were significantly different (p<0.05).},
DOI = {10.32604/phyton.2006.75.109}
}