@Article{phyton.2006.75.125,
AUTHOR = {García-Zambrano Eduardo A, Adriana  Gutiérrez-Díez, Gilberto E Salinas-García, Elizabeth  Cárdenas-Cerda, Rigoberto E  Vázquez-Alvarado, Francisco Zavala-García, Jesús Martínez de la  Cerda},
TITLE = {Classification and estimation of genetic diversity in Nopal Opuntia spp based on phenotypic descriptors and genetic-molecular markers},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {75},
YEAR = {2006},
NUMBER = {all},
PAGES = {125--135},
URL = {http://www.techscience.com/phyton/v75nall/36869},
ISSN = {1851-5657},
ABSTRACT = {The objective of this investigation was to develop and apply molecular markers (type RAPD) for estimating the genetic diversity of 100 <i>Opuntia accessions</i> from the Germoplasma Bank of the FAUANL. The molecular data were evaluated by multivariate analysis using the methods of Ward and UPGMA. These results were compared with phenotypic data taken from the same accessions, already evaluated by the above mentioned methods. The protocol was modified for the extraction of the <i>Opuntia</i> DNA to reduce the quantity of polysaccharides and their influence in its quality. Later on, a protocol was developed for the generation of molecular markers of the type RAPD for <i>Opuntia</i> accessions. This allowed to combine the accessions by the similarity degree of the molecular markers, in agreement with phenotype data.},
DOI = {10.32604/phyton.2006.75.125}
}