TY  - EJOU
AU  - García-Zambrano, 
AU  - Salinas-García, Gilberto E 
AU  - Cárdenas-Cerda, Elizabeth  
AU  - Vázquez-Alvarado, Rigoberto E  
AU  - Zavala-García, Francisco 
AU  - Cerda, Jesús Martínez de la  

TI  - Classification and estimation of genetic diversity in Nopal Opuntia spp based on phenotypic descriptors and genetic-molecular markers
T2  - Phyton-International Journal of Experimental Botany

PY  - 2006
VL  - 75
IS  - all
SN  - 1851-5657

AB  - The objective of this investigation was to develop and apply molecular markers (type RAPD) for estimating the genetic diversity of 100 <i>Opuntia accessions</i> from the Germoplasma Bank of the FAUANL. The molecular data were evaluated by multivariate analysis using the methods of Ward and UPGMA. These results were compared with phenotypic data taken from the same accessions, already evaluated by the above mentioned methods. The protocol was modified for the extraction of the <i>Opuntia</i> DNA to reduce the quantity of polysaccharides and their influence in its quality. Later on, a protocol was developed for the generation of molecular markers of the type RAPD for <i>Opuntia</i> accessions. This allowed to combine the accessions by the similarity degree of the molecular markers, in agreement with phenotype data.
KW  - molecular markers
KW  -  RAPD
KW  -  accesion
KW  -  PCR

DO  - 10.32604/phyton.2006.75.125