TY  - EJOU
AU  - Giménez, 
AU  - Haddad, O 

TI  - Genetic and resistance stability to Black Sigatoka disease during micropopagation of <i>Musa</i> CIEN BTA-03 somaclonal variant
T2  - Phyton-International Journal of Experimental Botany

PY  - 2008
VL  - 77
IS  - all
SN  - 1851-5657

AB  - Evaluation of clonal micropropagation and phenotype stability of elite somaclones are critical steps for development of new varieties. In the present work somaclon variant CIEN BTA-03 (resistant to Black Sigatoka), obtained through <i>in vitro</i> process from cultivar Williams (susceptible to Black Sigatoka), was micropropagated via apical shoot culture for five multiplication cycles in 0.5 mg/l of benzyl-aminopurine (BA). To verify the genetic stability of the progeny of this elite material, random amplified polymorphic DNA (RAPD) markers were used. A total of 5,292 monomorphic bands were obtained from the amplification of fifty six DNA samples (extracted from vitroplants randomly selected) with 10 different primer combinations. Non-polymorphic RAPD bands were found in this assay. Additionally, six plants of CIEN BTA-03 produced in the fifth cycle of micropropagation and the same number of plants from cultivars Pisang Mas, Williams and Yagambi Km5, were tested in the field to evaluate their response to Black Sigatoka disease. The clonally micropropagated CIEN BTA-03 plants preserved the Black Sigatoka resistance phenotype.
KW  - Micropropagation
KW  -  genetic stability
KW  -  RAPD
KW  -  disease resistance
KW  -  youngest leaf spotted (YLS)

DO  - 10.32604/phyton.2008.77.065