@Article{phyton.2010.79.005,
AUTHOR = {Lovey RJ, P  Perissé, C V  ieyra, JC  Coraglio},
TITLE = {Seed, germination and seedling characterization of <i>Cologania broussonetii</i> (Balb.) DC.},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {79},
YEAR = {2010},
NUMBER = {all},
PAGES = {5--10},
URL = {http://www.techscience.com/phyton/v79nall/36938},
ISSN = {1851-5657},
ABSTRACT = {<i>Cologania broussonetii</i> (Balb.) DC. (Fabaceae) grows from the Bolivian eastern Andes to the north west of Argentina, Cordoba hills and San Luis Province. It is recognized as a native forage. The objectives of this study were to characterize the seed, the germination and the seedling of <i>C. broussonetii</i>. Seed structure was characterized through free hand sections and analyzed with optical and stereoscopic microscopy. The germination assay treatments were: (1) intact non-scarfied seeds at 25 °C constant, (2) scarified seeds at 25 °C constant, (3) intact non-scarified seeds at 20-30 °C alternating, and (4) scarified seeds at 20-30 °C alternating. Results showed that seed is variable in shape depending upon its position within the legume. Seed coat is shiny, reddish brown with black streaks. The hilum is surrounded by a corona and it presents an aril with double hilar tongue. The micropyle is deltoid and the lens is visible. The embryo accumulates reserve substances and it is surrounded by a conspicuous endosperm layer. The embryo is in axile position investing subtype, the plumule is moderately developed and the radicle apex, protected by a radicle pocket, is triangular. Germination assays showed that scarified seeds exceeded 90% germination; meanwhile intact seeds did not reach the 50%. Maximum germination occurred at 20-30 °C, 15 days after seeding. Germination is phaneroepigeal; cotyledons present entire margin, the first pair of leaves is unifoliolated subtended by a pair of stipules. The following alternating leaves are trifoliolated and stipulated. Seedling morphology of <i>C. broussonetii</i> corresponds with the types Sloanea subtypes Sloanea and Sophora.},
DOI = {10.32604/phyton.2010.79.005}
}