@Article{phyton.2010.79.163,
AUTHOR = {Feng RJ, LF Lu, KH Yuan, P Cheng, LL Zhang, JF Qi, Y Ren, XL Xu, XB Zhang, LY Zhou, YD Zhang},
TITLE = {Cloning and expression analysis of rubredoxin from cold-treated banana leaves},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {79},
YEAR = {2010},
NUMBER = {all},
PAGES = {163--168},
URL = {http://www.techscience.com/phyton/v79nall/36958},
ISSN = {1851-5657},
ABSTRACT = {A banana (<i>Musa AAA</i>, Cavendish subgroup cv. Brazil) cDNA encoding a putative rubredoxin-like protein (<i>MaRd1</i>) was obtained from total RNA isolated from cold-treated banana leaves using rapid amplification of cDNA ends (RACE) technique. <i>MaRd1</i> cDNA contained 597 nucleotides encoding 198 amino acids in the open reading frame. MaRd1 protein showed 56% amino acid identity with that of <i>Pyrococcus furiosus</i> rubredoxin (P24297). A chloroplast transit peptide and a transmembrane region were detected at the N-terminus and the C-terminus, respectively, of the deduced amino acid sequence of <i>MaRd1</i> gene. Southern blotting revealed the occurrence of at least two copies of <i>MaRd1</i> in the banana genome. Real time quantitative RT-PCR analysis revealed that the expression of <i>MaRd1</i> gene was mainly in leaves, pseudo-stems and immature fruits, while it was barely detectable in roots and flowers. Cold and salt stresses induced higher levels of <i>MaRd1</i> transcript accumulation in leaves. This finding indicated a role of <i>MaRd1</i> in the response to these abiotic stresses.},
DOI = {10.32604/phyton.2010.79.163}
}