TY  - EJOU
AU  - Feng, 
AU  - Yuan, KH 
AU  - Cheng, P 
AU  - Zhang, LL 
AU  - Qi, JF 
AU  - Ren, Y 
AU  - Xu, XL 
AU  - Zhang, XB 
AU  - Zhou, LY 
AU  - Zhang, YD 

TI  - Cloning and expression analysis of rubredoxin from cold-treated banana leaves
T2  - Phyton-International Journal of Experimental Botany

PY  - 2010
VL  - 79
IS  - all
SN  - 1851-5657

AB  - A banana (<i>Musa AAA</i>, Cavendish subgroup cv. Brazil) cDNA encoding a putative rubredoxin-like protein (<i>MaRd1</i>) was obtained from total RNA isolated from cold-treated banana leaves using rapid amplification of cDNA ends (RACE) technique. <i>MaRd1</i> cDNA contained 597 nucleotides encoding 198 amino acids in the open reading frame. MaRd1 protein showed 56% amino acid identity with that of <i>Pyrococcus furiosus</i> rubredoxin (P24297). A chloroplast transit peptide and a transmembrane region were detected at the N-terminus and the C-terminus, respectively, of the deduced amino acid sequence of <i>MaRd1</i> gene. Southern blotting revealed the occurrence of at least two copies of <i>MaRd1</i> in the banana genome. Real time quantitative RT-PCR analysis revealed that the expression of <i>MaRd1</i> gene was mainly in leaves, pseudo-stems and immature fruits, while it was barely detectable in roots and flowers. Cold and salt stresses induced higher levels of <i>MaRd1</i> transcript accumulation in leaves. This finding indicated a role of <i>MaRd1</i> in the response to these abiotic stresses.
KW  - Rubredoxin
KW  -  Expression
KW  -  Banana
KW  -  Stress response

DO  - 10.32604/phyton.2010.79.163