@Article{phyton.2012.81.081,
AUTHOR = {Velázquez-Sepúlveda  I, MC  Orozco-Mosqueda, CM  Prieto-Barajas, G  Santoyo},
TITLE = {Bacterial diversity associated with the rhizosphere of wheat plants (<i>Triticum aestivum</i>): Toward a metagenomic analysis},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {81},
YEAR = {2012},
NUMBER = {all},
PAGES = {81--87},
URL = {http://www.techscience.com/phyton/v81nall/37010},
ISSN = {1851-5657},
ABSTRACT = {Rhizospheric soil is one the largest reservoirs of microbial genetic diversity. Before conducting a large-scale metagenomic analysis of an environment, such as a rhizospheric soil, it is necessary to perform a pre-screening of the resident genetic diversity. In this study, we analyzed the bacterial diversity associated with the rhizosphere of wheat plants by PCR amplification, construction of a library and sequencing of 16S rDNA genes. Thirty OTUs were detected, including the Classes Alfaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Gammaproteobateria, Actinobacteria, Bacilli, Clostridia and Uncultivable bacteria. Within the Gammaproteobacteria class, the genera <i>Pseudomonas, Stenotrophomonas</i> and <i>Bacillus</i> were the most abundant, since they corresponded to 40% of the whole ribosomal library. Phylogenetic analysis showed that most of the ribosomal sequences are grouped into clades that belong to common rhizospheric or bulk-soil bacteria. To determine whether the sample is significantly diverse, a Shannon-Wiener test was performed, resulting in a rate of 3.8 bits per individual. Our results suggest that the rhizosphere of wheat plants is highly diverse and results an excellent candidate for metagenomic analysis.},
DOI = {10.32604/phyton.2012.81.081}
}