TY  - EJOU
AU  - I, Velázquez-Sepúlveda  
AU  - Orozco-Mosqueda, MC  
AU  - Prieto-Barajas, CM  
AU  - Santoyo, G  

TI  - Bacterial diversity associated with the rhizosphere of wheat plants (<i>Triticum aestivum</i>): Toward a metagenomic analysis
T2  - Phyton-International Journal of Experimental Botany

PY  - 2012
VL  - 81
IS  - all
SN  - 1851-5657

AB  - Rhizospheric soil is one the largest reservoirs of microbial genetic diversity. Before conducting a large-scale metagenomic analysis of an environment, such as a rhizospheric soil, it is necessary to perform a pre-screening of the resident genetic diversity. In this study, we analyzed the bacterial diversity associated with the rhizosphere of wheat plants by PCR amplification, construction of a library and sequencing of 16S rDNA genes. Thirty OTUs were detected, including the Classes Alfaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Gammaproteobateria, Actinobacteria, Bacilli, Clostridia and Uncultivable bacteria. Within the Gammaproteobacteria class, the genera <i>Pseudomonas, Stenotrophomonas</i> and <i>Bacillus</i> were the most abundant, since they corresponded to 40% of the whole ribosomal library. Phylogenetic analysis showed that most of the ribosomal sequences are grouped into clades that belong to common rhizospheric or bulk-soil bacteria. To determine whether the sample is significantly diverse, a Shannon-Wiener test was performed, resulting in a rate of 3.8 bits per individual. Our results suggest that the rhizosphere of wheat plants is highly diverse and results an excellent candidate for metagenomic analysis.
KW  - Bacterial diversity
KW  -  Rhizosphere
KW  -  Wheat
KW  -  16S ribosomal genes

DO  - 10.32604/phyton.2012.81.081