@Article{phyton.2013.82.015,
AUTHOR = {Barocio-Ceja NB, LF Ceja-Torres, JL Morales-García, HV Silva-Rojas, R Flores-Magallón, S Ochoa-Estradav},
TITLE = {<i>In vitro</i> biocontrol of tomato pathogens using antagonists isolated from chicken-manure vermicompost},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {82},
YEAR = {2013},
NUMBER = {all},
PAGES = {15--22},
URL = {http://www.techscience.com/phyton/v82nall/37038},
ISSN = {1851-5657},
ABSTRACT = {The objectives of this study were to (1) isolate and identify pathogenic fungi from vegetative material with wilt symptoms in tomato plantations belonging to the Cienega of Chapala, Michoacán, Mexico, and (2) determine the antagonistic capacity of <i>Trichoderma</i> sp. and <i>Aspergillus</i> spp. isolated from chicken-manure vermicompost. Pathogens were isolated by means of a completely randomized sampling in 6 locations; 9 plantations were inspected and 45 plants with symptoms of the disease were selected. Portions of root and stem were disinfected and placed on potato-dextroseagar acidified (PDA). Antagonists isolation was made from a dilution of chicken-manure vermicompost of 1 x 10<sup>-2</sup> in PDA medium culture more streptomycin and tetracycline. The antagonistic activity was tested by the dual culture confrontation methods. Two pathogens were obtained on tomato in the study area, <i>Fusarium</i> spp. and <i>Rhizoctonia</i> sp., presenting an incidence of 92% and 5%, respectively. Morphological characteristics were determined in cultivation of PDA. Molecular analysis identified <i>F. oxysporum, F. solani, F. subglutinans</i> and <i>Rhizoctonia</i> sp. Of 11 isolates of chicken manure vermicompost, only <i>Trichoderma</i> sp. and <i>Aspergillus</i> sp., had significant differences (p≤0.05) with respect to the control. Inhibition of <i>F. oxysporum</i> growth ranged from 45% to 48%, and 24% to 27%, in presence of <i>Trichoderma</i> sp. and <i>Aspergillus</i> sp., respectively; these antagonistic species inhibited growth of <i>Rhizoctonia</i> sp. by 38% and 25% , respectively.},
DOI = {10.32604/phyton.2013.82.015}
}