@Article{phyton.2014.83.057,
AUTHOR = {Zhu XJ, YF Hu, X Chen, YH Wang, WP Fang, XH Li},
TITLE = {Endophytic fungi from <i>Camellia sinensis</i> show an antimicrobial activity against the rice blast pathogen <i>Magnaporthe grisea</i>},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {83},
YEAR = {2014},
NUMBER = {all},
PAGES = {57--63},
URL = {http://www.techscience.com/phyton/v83nall/37080},
ISSN = {1851-5657},
ABSTRACT = {The purpose of this study was to evaluate the antagonistic activity of two endophytic fungal strains, <i>Pseudocercospora kaki</i> and <i>Penicillium sclerotiorum</i>, isolated from the leaves of <i>Camellia sinensis</i>, against the rice blast pathogen <i>Magnaporthe grisea</i>. The inhibitory activity of the two endophytes against <i>M. grisea</i> in dual-culture was compared with that in monoculture. It was confirmed that the broth and its ethyl acetate extract of the dual-culture had a much stronger inhibition activity against <i>M. grisea</i> than the monocultures of <i>P. kaki</i> and <i>P. sclerotiorum</i>. The antagonism index of the broth and ethyl acetate extract from dual-culture to the mycelial growth of <i>M. grisea</i> was 78.02% ± 2.19% and 62.81% ± 2.29%, respectively, in different incubation periods. Qualitative analysis of ethyl acetate extract by GC-MS revealed that the number of bioactive compounds was greater in dual-culture than in monoculture. Compared to the ethyl acetate extract from monoculture, there were 10 constituents of bioactive compounds from dual-culture; however, there were only 6 types and 5 types from <i>P. kaki</i> monoculture and <i>P. sclerotiorum</i> monoculture, respectively. Glycerol; 4-Hydroxyphenyl ethanol; 1,2,3,4-Tetrahydroxy valeraldehyde and 1,2-Benzene-dicarboxylic acid dicyclohexyl ester were found in the ethyl acetate extracts of <i>P. kaki, P. sclerotiorum</i> and the dual culture. Based on the composition of the ethyl acetate extract of the dual culture of the endophytic fungi it might be possible to make a bioformulation for the biocontrol of the plant pathogen.},
DOI = {10.32604/phyton.2014.83.057}
}