@Article{phyton.2016.85.087,
AUTHOR = {Li RS, YJ Tao, FJ Liu, X Hu, QL Xu, KY Li},
TITLE = {<i>In vitro</i> plant regeneration via indirect organogenesis from different explants of <i>Lathyrus sativus</i> L. and <i>Lathyrus cicera</i> L.},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {85},
YEAR = {2016},
NUMBER = {all},
PAGES = {87--93},
URL = {http://www.techscience.com/phyton/v85nall/37193},
ISSN = {1851-5657},
ABSTRACT = {The grass pea (<i>Lathyrus sativus</i> L.) and flatpod peavine (<i>Lathyrus cicera</i> L.) are the most economically important and widely cultivated <i>Lathyrus</i> species. However, their utilization is limited due to the presence of their endogenous toxin β-<i>N</i>-oxalyl-L-α, β-diaminopropionic acid (β-ODAP). Thus, a β-ODAP free variety should be developed through some plant breeding technique like either mutational breeding or genetic-manipulation. In this circumstance, the plant regeneration of <i>Lathyrus</i> species becomes a bottleneck. In the present study, an efficient system for in vitro regeneration of <i>L. sativus</i> with high β-ODAP levels, and <i>L. cicera</i> with low β-ODAP levels, was developed from different explants (axillary buds, leaves and stems). At first, the green nodular calli were induced from sterile seedlings. It was found that the pre-culture of sterile seedlings with 15 mg/L 6-benzyladenine (BA) was necessary for <i>L. cicera</i>, but not for <i>L. sativus</i>. All of these calli were able to differentiate into adventitious shoot formation when cultured further. Among those explants, leaf segments were the optimum because of their easy obtainment and high regeneration efficiency (i.e., 66.48% in <i>L. sativus</i> and 62.13% in <i>L. cicera</i>). Furthermore, it was found that the pre-treatment would significantly improve the efficiency for nodular calli induction in both varieties, although it was easier on explants of <i>L. sativus</i> than on those of <i>L. cicera</i>. When these in vitro-derived plantlets of the two <i>Lathyrus</i> species were planted on half-strength Murashige and Skoog medium (MS) medium with α-naphthalene acetic acid (NAA) supplemented, 60% of them developed several roots. After being transplanted into soil, above 85% of each <i>Lathyrus</i> species grew well. The protocol would be useful for further expanding the propagation and <i>Agrobacterium</i>-mediated genetic transformation to obtain low β-ODAP varieties.},
DOI = {10.32604/phyton.2016.85.087}
}