@Article{phyton.2017.86.236, AUTHOR = {Xie LB, X Wang, M Peng, Y Zhou, LX Chen, LX Liu, YL Gao, YH Guo}, TITLE = {Comparative proteome analysis in hot pepper (Capsicum annuum L.) after space flight}, JOURNAL = {Phyton-International Journal of Experimental Botany}, VOLUME = {86}, YEAR = {2017}, NUMBER = {all}, PAGES = {236--245}, URL = {http://www.techscience.com/phyton/v86nall/36817}, ISSN = {1851-5657}, ABSTRACT = {Hot pepper (Capsicum annuum L.) is an important crop all over the world. To explore and identify differentially expressed proteins of hot pepper after space flight, three space-induced mutants (Y1, Y2 and Y3), which obtained new traits after space flight compared with their control lines (W1 and W2), were analyzed using comparative proteome analysis. In this study, leaf morphological characteristics of five kinds of hot pepper variations were evaluated by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Results showed that obvious changes of cellular structure were seen in space induced mutants. Thirty nine out of the 255 differentially expressed proteins were successfully sequenced. Among these 39 proteins, 31 were homologous with known proteins, and the others were set as a hypothetical protein. All identified proteins were further classified into six groups including protein metabolism, energy metabolism and photosynthesis. In conclusion, these findings will benefit for the research of mutagenic mechanisms at the level of proteomics, and provide an effective way for new hot pepper breeding.}, DOI = {10.32604/phyton.2017.86.236} }