TY - EJOU
AU - PA, Tantasawat
AU - Lunwongsa, K
AU - Linthaisong, T
AU - Wirikitgul, P
AU - Campatong, N
AU - Talpolkrung, N
AU - Tharapreuksapong, A
AU - Poolsawat, O
AU - Khairum, A
AU - Kativat, A Sorntip, C
TI - Genetic diversity of water primrose (Ludwigia hyssopifolia) in Thailand based on morphological characters and RAPD analysis
T2 - Phyton-International Journal of Experimental Botany
PY - 2018
VL - 87
IS - all
SN - 1851-5657
AB - Genetic diversity and relatedness of 17 water primrose
(Ludwigia hyssopifolia) accessions in Thailand were estimated using
morphological characters and random amplified polymorphic DNA
(RAPD) markers. Eight morphological characters were diverse
among the accessions. However, some accessions could not be distinguished
from one another based on these morphological characters
alone. Unweighted pair-group arithmetic average (UPGMA) analysis
of these characters separated these 17 accessions into 2 major clusters.
Among the 5 RAPD primers used, a total of 68 fragments (150
to 2000 bp) were amplified, showing a polymorphism percentage of
80%. The polymorphic information content (PIC) among accessions
varied from 0.31 to 0.36 with an average of 0.33. These polymorphic
RAPD markers successfully distinguished all 17 water primrose accessions.
The 17 accessions were grouped into 3 clusters using UPGMA
analysis. Pairwise coefficients of morphological characters and
RAPD-based genetic similarity between all accessions ranged from
0.154 to 1.000 with an average of 0.693 and from 0.531 to 0.952
with an average of 0.802, respectively. A nonsignificant correlation
between morphological characters and RAPD-based similarities was
found as shown by the low correlation coefficient of 0.242 between
the two Jaccard’s similarity matrices in the Mantel test. Clustering
of accessions within clusters also differed when morphological
characters and RAPD-derived dendrograms from UPGMA analysis
were compared. It would suggest that RAPD was more effective in
determining the genetic variability and relationships among water
primrose accessions. In addition, RAPD was also more useful for accessions
identification since all 17 water primrose accessions can be
effectively distinguished by only 5 RAPD primers.
KW - DNA markers; Genetic relationships; Random amplified polymorphic DNA; Seedbox
DO - 10.32604/phyton.2018.87.162