@Article{phyton.2019.04549,
AUTHOR = {Quezada-Rivera JJ, RE Soria-Guerra, FS Pérez-Juárez, L Martínez-González, SE Valdés- Rodríguez, NL Vasco-Méndez, JF Morales-Domínguez},
TITLE = {Heterologous expression of bacteriocin E-760 in Chlamydomonas reinhardtii and functional analysis},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {88},
YEAR = {2019},
NUMBER = {1},
PAGES = {25--35},
URL = {http://www.techscience.com/phyton/v88n1/33383},
ISSN = {1851-5657},
ABSTRACT = {The use of antimicrobial peptides (AMPs) synthesized
by bacteria (bacteriocins) is an alternative for combating multidrug
resistant bacterial strains and their production by recombinant route
is a viable option for their mass production. The bacteriocin E-760
isolated from the genus Enterococcus sp. has been shown to possess
inhibitory activity against Gram-negative and Gram-positive
bacteria. In this study, the expression of a chimeric protein coding
for E-760 in the nucleus of C. reinhardtii was evaluated, as well as,
its antibacterial activity. The synthetic gene E-760S was inserted
into the genome of C. reinhardtii using Agrobacterium tumefaciens.
A transgenic line was identified in TAP medium with hygromycin
and also by PCR. The increment in the culture medium temperature
of the transgenic strain at 35 °C for 10 minutes, increased the
production level of the recombinant protein from 0.14 (Noninduced
culture, NIC) to 0.36% (Induced culture, IC) of total soluble
proteins (TSP); this was quantified by an ELISA assay. Recombinant
E-760 possesses activity against Staphylococcus aureus in 0.34 U
log, Streptococcus agalactiae in 0.48 U log, Enterococcus faecium in
0.36 U log, Pseudomonas aeruginosa in 2 U log and for Klebsiella
pneumoniae, the activity was 0.07 U log. These results demonstrate
that the nucleus transformation of C. reinhardtii can function as
a stable expression platform for the production of the synthetic
gene E-760 and it can potentially be used as an antibacterial agent.},
DOI = {10.32604/phyton.2019.04549}
}