@Article{phyton.2019.05036,
AUTHOR = {Grisel Fierros-Romero, M. Elena Mellado-Rojas, Reynaldo C. Pless, Juan Ignacio Valiente-Banuet, Ashutosh Sharma and Elda M. Beltrán-Peña},
TITLE = {Torin 1, TOR Inhibitor Enhances Cellular Proliferation in NT-1 Tobacco Suspension Cell Cultures},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {88},
YEAR = {2019},
NUMBER = {2},
PAGES = {131--137},
URL = {http://www.techscience.com/phyton/v88n2/33403},
ISSN = {1851-5657},
ABSTRACT = {Torin 1 is an ATP-competitive TOR inhibitor which inhibits the signaling of TOR and S6K kinase in mammals and plants. The objective of this research is to determine the effect of Torin 1 in a relatively simple and homogeneous plant system such as the NT-1 tobacco suspension cell cultures. Cultures of NT-1 cells were tested with 5, 50, 150 and 250 nM of Torin 1. During kinetics growth of NT-1 tobacco suspension cell cultures, 150 and 250 nM Torin 1 inhibits the early growth and later enhanced the cellular proliferation during exponential growth by means of an increased expression of E2F1 and cyclin B. Furthermore, Torin 1 stimulates the growth of NT-1 cells during log phase with small shaped cell, characteristic of tobacco suspension cell cultures with high mitotic activity.},
DOI = {10.32604/phyton.2019.05036}
}