TY  - EJOU
AU  - Ovando-Domínguez, M. Y. 
AU  - Luján-Hidalgo, M. C. 
AU  - González-Mendoza, D. 
AU  - Vargas-Díaz, A. A. 
AU  - Ruiz-Lau, N. 
AU  - Gutiérrez-Miceli, F. A. 
AU  - Lecona-Guzmán, C. A. 

TI  - Total Phenols, Flavonoids and Antioxidant Activity in <i>Annona muricata</i> and <i>Annona purpurea</i> Callus Culture
T2  - Phyton-International Journal of Experimental Botany

PY  - 2019
VL  - 88
IS  - 2
SN  - 1851-5657

AB  - Callus cultures of Annona muricata and Annona purpurea were induced in Murashige and Skoog (MS) medium supplemented with different concentrations of 1-naphthylacetic acid (NAA), 6-benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D) utilized hypocotyls with explant. The highest percentage of callus formation was the treatment supplemented with 3 mg L<sup>-1</sup> NAA for <i>A. muricata</i> (100%) while for <i>A. purpurea</i> in lower percentage (75%). BA stimulated the formation of shoots in all the evaluated concentrations, being the concentration of 2 mg L<sup>-1</sup> the one that induced the greater formation of shoots for <i>A. muricata</i> (23 shoots/explant) and <i>A. purpurea</i> (28 shoots/explant). The content of total phenols, flavonoids and antioxidant activity was measured in the callus obtained from both species. The results showed that a higher content of total phenols was quantified in callus of <i>A. purpurea</i> (27.8 mg g<sup>-1</sup> dw) compared to <i>A. muricata</i> (23.2 mg g<sup>-1</sup> dw). The highest content of total flavonoids was observed in the callus of <i>A. purpurea</i> (8.0 μg g<sup>-1</sup> dw). Antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydracil radical assay. The concentration required for 50% inhibition (IC50) of the 2,2-diphenyl-1-picrylhydracil radicals were 4.22 μg mL<sup>-1</sup> in methanolic extracts of callus of <i>A. muricata</i>, while in extracts of callus of <i>A. purpurea</i> was 2.86 μg mL<sup>-1</sup>, in both cases was greater than that found for leaves. Callus culture of the species studied in this work represents an alternative for the production of natural antioxidants.
KW  - Annonaceae; antioxidant activity; 6-benzyladenine; secondary metabolites; tissue culture

DO  - 10.32604/phyton.2019.06546