@Article{phyton.2020.07835,
AUTHOR = {Aarón Giovanni Munguía-Rodríguez, José López-Bucio, Gerardo Rangel Sánchez, León Francisco Ruiz-Herrera, Yazmín Carreón-Abud, Miguel Martínez-Trujillo},
TITLE = {Chromium Differentially Affects Hydrogen Peroxide Distribution in Primary and Adventitious Roots of <i>Arabidopsis thaliana</i> L.},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {89},
YEAR = {2020},
NUMBER = {1},
PAGES = {35--43},
URL = {http://www.techscience.com/phyton/v89n1/38397},
ISSN = {1851-5657},
ABSTRACT = {The post-embryonic growth of the <i>Arabidopsis thaliana</i> root system can 
be modified by different types of stress, such as sublethal concentrations of metals, 
which may induce the production of reactive oxygen species (ROS). In this study,
the effects of different concentrations of potassium chromate (K<sub>₂</sub>CrO<sub>4</sub>) on the 
distribution and relative quantity of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) were determined in
primary and adventitious roots in <i>A. thaliana</i> HyPer line seedlings. This line has a 
biosensor that specifically reports H<sub>2</sub>O<sub>2</sub> levels within tissues as fluorescence. 
Primary root growth was inhibited at 100 μM Cr (VI); in contrast, adventitious root
formation was induced over the main root growth axis. These structures 
proliferated from 100-160 μM Cr (VI), and much higher concentrations (180-200 
μM) of K<sub>₂</sub>CrO<sub>4</sub> were required to affect their growth. The H<sub>2</sub>O<sub>2</sub> distributions were 
observed in the columella and lateral root cap of primary roots of plants grown in 
medium lacking dichromate, but following the development of toxicity symptoms, H<sub>2</sub>O<sub>2</sub> changed its distribution to the meristem and differentiation zones. Conversely, 
adventitious roots had comparable H<sub>2</sub>O<sub>2</sub> distribution patterns in untreated plants
and those exposed to Cr (VI) supplementation. Thus, differential H<sub>2</sub>O<sub>2</sub> distribution 
correlates with the resistance of adventitious roots, but not primary roots, to 
dichromate and underlies cell reorganization at the apex to support growth.},
DOI = {10.32604/phyton.2020.07835}
}