@Article{phyton.2020.09334,
AUTHOR = {Jingsen Liu, Jinqi Ma, Ai Lin, Chao Zhang, Bo Yang, Liyuan Zhang, Lin Huang, Jiana Li},
TITLE = {Genome-Wide Identification and Expression Profiling Suggest that Invertase Genes Function in Silique Development and the Response to <i>Sclerotinia sclerotiorum</i> in <i>Brassica napus</i>},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {89},
YEAR = {2020},
NUMBER = {2},
PAGES = {253--273},
URL = {http://www.techscience.com/phyton/v89n2/38671},
ISSN = {1851-5657},
ABSTRACT = {Invertase (INV), a key enzyme in sucrose metabolism, irreversibly catalyzes the hydrolysis of sucrose to glucose and fructose, thus playing important
roles in plant growth, development, and biotic and abiotic stress responses. In this
study, we identified 27 members of the <i>BnaINV</i> family in <i>Brassica napus</i>. We
constructed a phylogenetic tree of the family and predicted the gene structures,
conserved motifs, <i>cis</i>-acting elements in promoters, physicochemical properties
of encoded proteins, and chromosomal distribution of the <i>BnaINV</i>s. We also analyzed the expression of the <i>BnaINV</i>s in different tissues and developmental stages
in the <i>B. napus</i> cultivar Zhongshuang 11 using qRT-PCR. In addition, we analyzed RNA-sequencing data to explore the expression patterns of the <i>BnaINV</i>s
in four cultivars with different harvest indices and in plants inoculated with the
pathogenic fungus <i>Sclerotinia sclerotiorum</i>. We used WGCNA (weighted coexpression network analysis) to uncover <i>BnaINV</i>regulatory networks. Finally,
we explored the expression patterns of several <i>BnaINV</i> genes in cultivars with
long (Zhongshuang 4) and short (Ningyou 12) siliques. Our results suggest that
BnaINVs play important roles in the growth and development of rapeseed siliques
and the defense response against pathogens. Our findings could facilitate the
breeding of high-yielding <i>B. napus</i> cultivars with strong disease resistance.},
DOI = {10.32604/phyton.2020.09334}
}